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LCMV‐infected Znfx1 mut mice show T cell expansion and more pronounced Th1 polarisation. (A) The white blood cell (WBC) count in whole blood. (B) The T cell count in whole blood. (C) Flow cytometry plot of CD62L and CD44 expression on CD4 + T cells in the spleen. EM: effector memory. (D) Flow cytometry plot of Ki‐67 expression on CD4 + T cells in the spleen. (E) Flow cytometry plot of T‐bet expression in CD4 + T cells in the spleen. (F) Flow cytometry plot of CXCR3 expression on CD4 + T cells in the spleen. (G) Flow cytometry plot of IFN‐γ expression in CD4 + T cells taken from the spleen after in vitro stimulation with 0.08 µM <t>phorbol</t> <t>12‐myristate</t> <t>13‐acetate,</t> 1.34 µM ionomycin, and brefeldin A for 5 h. (H) Flow cytometry plot of CXCR3 expression on CD8 + T cells in the spleen. (I) Flow cytometry plot of IFN‐γ expression in CD8 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (J) Flow cytometry plot of IFN‐γ expression in CD8 + T cells after in vitro stimulation with 10 µM gp33‐41 and brefeldin A for 5 h. All plots show cells from mice mock‐infected with NaCl or 9 days post infection (p.i.) with 200 PFU of LCMV strain WE. The data were analyzed in a one‐way analysis of variance with Šidák's correction for multiple comparisons. The graphs display the mean and the standard deviation. Each dot represents an individual mouse, and (except for the T cell count in blood) all plots show the data from at least two independent experiments. p ‐values are indicated above the plots.
Phorbol 12 Myristate 13 Acetate Pma, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LCMV‐infected Znfx1 mut mice show T cell expansion and more pronounced Th1 polarisation. (A) The white blood cell (WBC) count in whole blood. (B) The T cell count in whole blood. (C) Flow cytometry plot of CD62L and CD44 expression on CD4 + T cells in the spleen. EM: effector memory. (D) Flow cytometry plot of Ki‐67 expression on CD4 + T cells in the spleen. (E) Flow cytometry plot of T‐bet expression in CD4 + T cells in the spleen. (F) Flow cytometry plot of CXCR3 expression on CD4 + T cells in the spleen. (G) Flow cytometry plot of IFN‐γ expression in CD4 + T cells taken from the spleen after in vitro stimulation with 0.08 µM <t>phorbol</t> <t>12‐myristate</t> <t>13‐acetate,</t> 1.34 µM ionomycin, and brefeldin A for 5 h. (H) Flow cytometry plot of CXCR3 expression on CD8 + T cells in the spleen. (I) Flow cytometry plot of IFN‐γ expression in CD8 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (J) Flow cytometry plot of IFN‐γ expression in CD8 + T cells after in vitro stimulation with 10 µM gp33‐41 and brefeldin A for 5 h. All plots show cells from mice mock‐infected with NaCl or 9 days post infection (p.i.) with 200 PFU of LCMV strain WE. The data were analyzed in a one‐way analysis of variance with Šidák's correction for multiple comparisons. The graphs display the mean and the standard deviation. Each dot represents an individual mouse, and (except for the T cell count in blood) all plots show the data from at least two independent experiments. p ‐values are indicated above the plots.
Pma, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LCMV‐infected Znfx1 mut mice show T cell expansion and more pronounced Th1 polarisation. (A) The white blood cell (WBC) count in whole blood. (B) The T cell count in whole blood. (C) Flow cytometry plot of CD62L and CD44 expression on CD4 + T cells in the spleen. EM: effector memory. (D) Flow cytometry plot of Ki‐67 expression on CD4 + T cells in the spleen. (E) Flow cytometry plot of T‐bet expression in CD4 + T cells in the spleen. (F) Flow cytometry plot of CXCR3 expression on CD4 + T cells in the spleen. (G) Flow cytometry plot of IFN‐γ expression in CD4 + T cells taken from the spleen after in vitro stimulation with 0.08 µM <t>phorbol</t> <t>12‐myristate</t> <t>13‐acetate,</t> 1.34 µM ionomycin, and brefeldin A for 5 h. (H) Flow cytometry plot of CXCR3 expression on CD8 + T cells in the spleen. (I) Flow cytometry plot of IFN‐γ expression in CD8 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (J) Flow cytometry plot of IFN‐γ expression in CD8 + T cells after in vitro stimulation with 10 µM gp33‐41 and brefeldin A for 5 h. All plots show cells from mice mock‐infected with NaCl or 9 days post infection (p.i.) with 200 PFU of LCMV strain WE. The data were analyzed in a one‐way analysis of variance with Šidák's correction for multiple comparisons. The graphs display the mean and the standard deviation. Each dot represents an individual mouse, and (except for the T cell count in blood) all plots show the data from at least two independent experiments. p ‐values are indicated above the plots.
Phorbol 12 Myristate 13 Acetate Pma, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pma  (MedChemExpress)
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MedChemExpress pma
LCMV‐infected Znfx1 mut mice show T cell expansion and more pronounced Th1 polarisation. (A) The white blood cell (WBC) count in whole blood. (B) The T cell count in whole blood. (C) Flow cytometry plot of CD62L and CD44 expression on CD4 + T cells in the spleen. EM: effector memory. (D) Flow cytometry plot of Ki‐67 expression on CD4 + T cells in the spleen. (E) Flow cytometry plot of T‐bet expression in CD4 + T cells in the spleen. (F) Flow cytometry plot of CXCR3 expression on CD4 + T cells in the spleen. (G) Flow cytometry plot of IFN‐γ expression in CD4 + T cells taken from the spleen after in vitro stimulation with 0.08 µM <t>phorbol</t> <t>12‐myristate</t> <t>13‐acetate,</t> 1.34 µM ionomycin, and brefeldin A for 5 h. (H) Flow cytometry plot of CXCR3 expression on CD8 + T cells in the spleen. (I) Flow cytometry plot of IFN‐γ expression in CD8 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (J) Flow cytometry plot of IFN‐γ expression in CD8 + T cells after in vitro stimulation with 10 µM gp33‐41 and brefeldin A for 5 h. All plots show cells from mice mock‐infected with NaCl or 9 days post infection (p.i.) with 200 PFU of LCMV strain WE. The data were analyzed in a one‐way analysis of variance with Šidák's correction for multiple comparisons. The graphs display the mean and the standard deviation. Each dot represents an individual mouse, and (except for the T cell count in blood) all plots show the data from at least two independent experiments. p ‐values are indicated above the plots.
Pma, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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LCMV‐infected Znfx1 mut mice show T cell expansion and more pronounced Th1 polarisation. (A) The white blood cell (WBC) count in whole blood. (B) The T cell count in whole blood. (C) Flow cytometry plot of CD62L and CD44 expression on CD4 + T cells in the spleen. EM: effector memory. (D) Flow cytometry plot of Ki‐67 expression on CD4 + T cells in the spleen. (E) Flow cytometry plot of T‐bet expression in CD4 + T cells in the spleen. (F) Flow cytometry plot of CXCR3 expression on CD4 + T cells in the spleen. (G) Flow cytometry plot of IFN‐γ expression in CD4 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (H) Flow cytometry plot of CXCR3 expression on CD8 + T cells in the spleen. (I) Flow cytometry plot of IFN‐γ expression in CD8 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (J) Flow cytometry plot of IFN‐γ expression in CD8 + T cells after in vitro stimulation with 10 µM gp33‐41 and brefeldin A for 5 h. All plots show cells from mice mock‐infected with NaCl or 9 days post infection (p.i.) with 200 PFU of LCMV strain WE. The data were analyzed in a one‐way analysis of variance with Šidák's correction for multiple comparisons. The graphs display the mean and the standard deviation. Each dot represents an individual mouse, and (except for the T cell count in blood) all plots show the data from at least two independent experiments. p ‐values are indicated above the plots.

Journal: European Journal of Immunology

Article Title: A Novel Murine Model of Hemophagocytic Lymphohistiocytosis‐Like Inflammation in ZNFX1 Deficiency

doi: 10.1002/eji.70141

Figure Lengend Snippet: LCMV‐infected Znfx1 mut mice show T cell expansion and more pronounced Th1 polarisation. (A) The white blood cell (WBC) count in whole blood. (B) The T cell count in whole blood. (C) Flow cytometry plot of CD62L and CD44 expression on CD4 + T cells in the spleen. EM: effector memory. (D) Flow cytometry plot of Ki‐67 expression on CD4 + T cells in the spleen. (E) Flow cytometry plot of T‐bet expression in CD4 + T cells in the spleen. (F) Flow cytometry plot of CXCR3 expression on CD4 + T cells in the spleen. (G) Flow cytometry plot of IFN‐γ expression in CD4 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (H) Flow cytometry plot of CXCR3 expression on CD8 + T cells in the spleen. (I) Flow cytometry plot of IFN‐γ expression in CD8 + T cells taken from the spleen after in vitro stimulation with 0.08 µM phorbol 12‐myristate 13‐acetate, 1.34 µM ionomycin, and brefeldin A for 5 h. (J) Flow cytometry plot of IFN‐γ expression in CD8 + T cells after in vitro stimulation with 10 µM gp33‐41 and brefeldin A for 5 h. All plots show cells from mice mock‐infected with NaCl or 9 days post infection (p.i.) with 200 PFU of LCMV strain WE. The data were analyzed in a one‐way analysis of variance with Šidák's correction for multiple comparisons. The graphs display the mean and the standard deviation. Each dot represents an individual mouse, and (except for the T cell count in blood) all plots show the data from at least two independent experiments. p ‐values are indicated above the plots.

Article Snippet: The cells were stimulated with 10 μM gp33‐41 (Med Chem Express), 10 μM gp61‐80 (Med Chem Express), or 0.08 μM phorbol 12‐myristate 13‐acetate (PMA), and 1.34 μM ionomycin (eBioscience Cell Stimulation Cocktail, Thermo Scientific).

Techniques: Infection, Cell Characterization, Flow Cytometry, Expressing, In Vitro, Standard Deviation